Functional
Analysis of SpltMNPV Envelope Protein SL136
ZHANG Ping,PANG Yi*,YANG Bo,LI
Zhao-Fei,YIN Chong,SU De-Ming1
( State Key Laboratory for Biocontrol,Zhongshan University,Guangzhou 510275,China;
1Virology Research Unit,Fudan University,Shanghai 200433,China
)
Abstract Our
previous research showed that the Spodoptera litura multinucleocapsid
nucleopolyhedrovirus (SpltMNPV) Sl136 product had a function to befused with membrane when
expressed alone.In this study, the Sl136 and its product were
characterized. RT-PCR results showed that Sl136 could be transcribed at
6 hpost infection, indicating it was an early gene.Then the antiserum against
SL136 protein was generated and utilized to verify that SL136 was a BV envelope
protein, by using SDS-PAGE and Western blot.Two main protein bands in
SpltMNPV-infected Sl-zsu-1 cells were detected;their molecular weights were
about 86 kD and 65 kD,respectively.It was found that the size of smaller band
coincided with amajor band of BV envelope proteins.SL136 protein was
transferred to cell surfaces both in SpltMNPV-infected Sl-zsu-1 cells and
recombinant Bac-Sl136-infected Hi5 cells,as detected by cell ELISA£¨cell enzyme-linked
immunosorbant assay,CELISA).From the bioassay results,it was found that furin
cleavage of SL136 was not necessary for viral propagation,and inhibition of its
glycosylation decreased the BV virulence.
Key words SpltMNPV; budded virus; envelope protein
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e-mail, [email protected]